| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 51 | Leptospira biflexa | AGGGACAGAAGCTGCGAT | CCGGTTTTCCATGGCATTGG | 58.28 | 60.11 | 281 | 130 |
100.00%
|
100.00%
|
| Primer 52 | Leptospira biflexa | GCGATGAAGGAAGGAGGGAC | CCGGTTTTCCATGGCATTGG | 60.18 | 60.11 | 295 | 130 |
100.00%
|
100.00%
|
| Primer 53 | Leptospira biflexa | AGACGGAGAAGGTAAGTTCGG | GGGCTCTTTGGTGTTTGCC | 59.18 | 59.63 | 191 | 130 |
100.00%
|
100.00%
|
| Primer 54 | Leptospira biflexa | ACGACCATCCAACCGCAA | TGGTCTGCCACAAATCCCA | 59.57 | 59.15 | 271 | 130 |
100.00%
|
100.00%
|
| Primer 55 | Leptospira biflexa | ACGACCATCCAACCGCAA | AGGCTGGGACATCAAGAACG | 59.57 | 60.04 | 219 | 130 |
100.00%
|
100.00%
|
| Primer 56 | Leptospira biflexa | ACGACCATCCAACCGCAA | TCCAGGAAGGCATCGTAACG | 59.57 | 59.83 | 163 | 130 |
100.00%
|
100.00%
|
| Primer 57 | Leptospira biflexa | ACGACCATCCAACCGCAA | TGGTCTGCCACAAATCCCAT | 59.57 | 59.59 | 271 | 130 |
100.00%
|
100.00%
|
| Primer 58 | Leptospira biflexa | ACGAATGCAACCAAACGGAC | GCCAGGCGATTGCAAAGT | 59.69 | 59.04 | 188 | 130 |
100.00%
|
100.00%
|
| Primer 59 | Leptospira biflexa | TGGGCAAAGGTGAGGACAC | TTTGAGCCTAGCGAGTCCC | 59.85 | 59.10 | 251 | 129 |
100.00%
|
100.00%
|
| Primer 60 | Leptospira biflexa | TGGGCAAAGGTGAGGACAC | TTTTGAGCCTAGCGAGTCCC | 59.85 | 59.75 | 252 | 129 |
100.00%
|
100.00%
|